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Expression of biomineralisation genes in tissues and cultured cells of the abalone Haliotis tuberculata

TitreExpression of biomineralisation genes in tissues and cultured cells of the abalone Haliotis tuberculata
Type de publicationJournal Article
Year of Publication2013
AuteursNeill, MO', Gaume, B, Denis, F, Auzoux-Bordenave, S
JournalCytotechnology
Volume65
Ticket5
Pagination737-747
Date Published2013
Type of ArticleResearch article
Mots-clésbiomineralisation, Gene Expression, Haliotis tuberculata, primary culture
Résumé

Mollusc shell biomineralisation involves a variety of organic macromolecules (matrix proteins and enzymes) that control CaCO3 deposition, growth of crystals, the selection of polymorph, and the microstructure of the shell. Since the mantle and the hemocytes play an important role in the control of shell formation, primary cell cultures have been developed to study the expression of three biomineralisation genes recently identified in the abalone Haliotis tuberculata: a matrix protein, Lustrin A, and two carbonic anhydrase enzymes. Mantle cells and hemocytes were successfully maintained in primary cultures and were evaluated for their viability and proliferation over time using semi-automated assay (XTT). PCR and gel photograph analysis were used to semi-quantify the gene expression and compare the level of expression in native tissues and cultured cells. The results demonstrated that the three genes of interest were being expressed in abalone tissues, with expression highest in the mantle and much lower in the hemocytes and the gills. Biomineralisation genes were also expressed significantly in mantle cells, confirming that primary cultures of target tissues are suitable models for in vitro investigation of matrix protein secretion.